SARS-CoV-2 Spike-Membrane Recombinant Fusion Protein (Bioactivity Verified)
CAT#: TP701119
Purified recombinant fusion protein that includes the RBD of SARS-CoV-2 Spike protein (S-Protein) and Virion Surface Domain of Membrane Protein (M-protein) with C-terminal HIS-tag
View other "S Protein" proteins (19)
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Avi-tag Biotinylated Protein Get a free quote |
CNY 2,900.00
CNY 7,490.00
Cited in 1 publication. |
CNY 1,999.00
CNY 3,600.00
Specifications
Product Data | |
Species | SARS-CoV-2 |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
MALWIDRMQL LSCIALSLAL VTNSAKGIYQ TSNFRVQPTE SIVRFPNITN LCPFGEVFNA
TRFASVYAWN RKRISNCVAD YSVLYNSASF STFKCYGVSP TKLNDLCFTN VYADSFVIRG DEVRQIAPGQ TGKIADYNYK LPDDFTGCVI AWNSNNLDSK VGGNYNYLYR LFRKSNLKPF ERDISTEIYQ AGSTPCNGVE GFNCYFPLQS YGFQPTNGVG YQPYRVVVLS FELLHAPATV CGPKKSTNLV KNKCVNFNFN GLTGTGVLTE SNKKFLGGGS GGGSMADSNG TITVEELKKH HHHHH |
Tag | C-HIS |
Predicted MW | 34 kDa |
Concentration | >0.05 µg/µL as determined by Bradford protein assay method. |
Purity | > 90% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | PBS, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Bioactivity | Binding of SARS-CoV-2 S-M Fusion Protein to Human ACE2: 2 ug/ml of SARS-CoV-2 Spike and Membrane viral fusion protein (gel insert) was immobilized on an ELISA plate and its ability to bind to Human ACE2 was measured. The EC50 was between 10-20 ng/ml. |
Reference Data |
Citations (1)
The use of this Proteins has been cited in the following citations: |
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SARS-CoV-2/ACE2 Interaction Suppresses IRAK-M Expression and Promotes Pro-Inflammatory Cytokine Production in Macrophages
,null,
Frontiers in Immunology
,PubMed ID 34248968
[S-M protein]
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