Thioredoxin / TRX1 (1-105) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
Predicted MW	11.7 kDa
Concentration	lot specific
Purity	≥90% by SDS PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: PBS, pH 7.4
Preparation	Liquid purified protein
Applications	Protocol: Activity Assay 1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately. 3. Incubate at 25°C for 20 minutes. 4. Record the increase in A650nm for 15 minutes
Protein Description	Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin. Please refer to our activity assay protocol below.
Reference Data
RefSeq	NP_001231867
Locus ID	7295
UniProt ID	P10599
Cytogenetics	9q31.3
Synonyms	TXN, TRDX, TRX, ATL-derived factor, ADF, SASP
Summary	The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Protein Families	Protocol: Activity Assay 1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately. 3. Incubate at 25°C for 20 minutes. 4. Record the increase in A650nm for 15 minutes

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