PPIL1 (1-166, His-tag) Human Protein
CAT#: AR09117PU-N
PPIL1 (1-166, His-tag) human recombinant protein, 0.1 mg
Need it in bulk or customized? Get a free quote |
CNY 8,000.00
货期*
详询
规格
Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPDL KFTGAGILAM ANAGPDTNGS QFFVTLAPTQ WLDGKHTIFG RVCQGIGMVN RVGMVETNSQ DRPVDDVKII KAYPSGLEHH HHHH
|
Tag | His-tag |
Predicted MW | 19.3 kDa |
Concentration | lot specific |
Purity | >95% by SDS PAGE |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl pH 8.0, 20% glycerol |
Preparation | Liquid purified protein |
Applications | Protocol: Activity Assay 1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin. 2. Add 10 ul of recombinant PPIL1 protein with 1 ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer. 4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM) 5. Record the increase in A405 nm for 30 minutes at 25°C. |
Protein Description | Recombinant PPIL1, fused to His-tag at C-terminus, was expressed in E.coli and purified by conventional chromatography techniques. |
Storage | Store (in aliquots) at -20°C or -70°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Bioactivity | Specific: > 300 nmoles/min/mg, defined as the amount of enzyme that cleaves 1 umole of suc-AAPF-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin |
Reference Data | |
RefSeq | NP_057143 |
Locus ID | 51645 |
UniProt ID | Q9Y3C6, A0A024RCX8, A0A494C014 |
Cytogenetics | 6p21.2 |
Synonyms | CGI-124; CYPL1; hCyPX; PCH14; PPIase |
Summary | This gene is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved, ubiquitous family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. Based on similarity to other PPIases, this protein could accelerate the folding of proteins and might catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. [provided by RefSeq, Jul 2008] |
Protein Pathways | Spliceosome |
Documents
FAQs |
SDS |
Customer
Reviews
Loading...