DHFR / DHFRP1 (1-187, His-tag) Mouse Protein
CAT#: AR09852PU-L
DHFR / DHFRP1 (1-187, His-tag) mouse recombinant protein, 0.5 mg
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CNY 14,920.00
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规格
Specifications
Product Data | |
Species | Mouse |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD
|
Tag | His-tag |
Predicted MW | 23.8 kDa |
Concentration | lot specific |
Purity | >95% |
Buffer | Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2 mM DTT, 0.1M NaCl |
Preparation | Liquid purified protein |
Protein Description | Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Bioactivity | Specific: > 0.2 units/mg, in which one unit will convert 1.0 umole of 7,8– dihydrofloate and beta - NADPH to 5,6,7,8 - tetrahydrofloate and beta - NADP per min at pH 6.5 at 25°C Activity Assay: 1. Prepare a 1.55 ml assay buffer. The final concentrations are 50 mM potassium phosphate, 0.072 mM dihydrofolic acid, 0.1 mM beta-nicotinamide dinucleotide phosphate and 0.003 % (w/v) bovine serum albumin. 2. Add 50 ul of recombinant DHFR protein in various concentrations (2 ug, 5 ug) in assay buffer. 3. Mix by inversion and record the decrease at A340nm for 5 minutes. |
Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
Reference Data | |
RefSeq | NP_034179 |
Locus ID | 13361 |
UniProt ID | P00375, Q544T5 |
Cytogenetics | 13 47.64 cM |
Synonyms | 8430436I03Rik; AA607882; AI662710; AW555094 |
Summary | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway (PubMed:25980602). Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (PubMed:25980602). Binds its own mRNA.[UniProtKB/Swiss-Prot Function] |
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