CD202b / TEK (C-term, His-tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	Insect
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) AMDLILINSL PLVSDAETSL TCIASGWRPH EPITIGRDFE ALMNQHQDPL EVTQDVTREW AKKVVWKREK ASKINGAYFC EGRVRGEAIR IRTMKMRQQA SFLPATLTMT VDKGDNVNIS FKKVLIKEDA VIYKNGSFIH SVPRHEVPDI LEVHLPHAQP QDAGVYSARY IGGNLFTSAF TRLIVRRCEA QKWGPECNHL CTACMNNGVC HEDTGECICP PGFMGRTCEK ACELHTFGRT CKERCSGQEG CKSYFCLPDP YGCSCATGWK GLQCNEACHP GFYGPDCKLR CSCNNGEMCD RFQGCLCSPG WQGLQCEREG IPRMTPKIVD LPDHIEVNSG KFNPICKASG WPLPTNEEMT LVKPDGTVLH PKDFNHTDHF SAIFTIHRIL PPDSGVWVCS VNTVAGMVEK PFNISVKVLP KPLNAPNVID TGHNFAVINI SSEPYFGD GPIKSKKLLY KPVNHYEAWQ HIQVTNEIVT LNYLEPRTEY ELCVQLVRRG EGGEGHPGPV RRFTTASIGL PPPRGLNLLP KSQTTLNLTW QPIFPSSEDD FYVEVERRSV QKSDQQNIKV PGNLTSVLNN LHPREQYVVR ARVNTKAQGE WSEDLTAWTL SDILPPQPEN IKISNITHSS AVISWTILDG YSISSITIRY KVQGKNEDQH VDVKIKNATI IQYQLKGLEP ETAYQVDIFA ENNIGSSNPA FSHEVTLPES QAPADLGGGK TRHHHHHH
Tag	His-tag
Predicted MW	95 kDa
Purity	>90%
Buffer	Presentation State: Purified State: Lyophilized purified protein. Buffer System: PBS without stabilizer
Preparation	Lyophilized purified protein.
Protein Description	Recombinant Human soluble TIE-2/TEK was fused with a 6x His-tag at the C-terminus. The soluble receptor protein consists of the full extracellular domain (Thr19-Lys745).
Storage	Lyophilized samples are stable for greater than six months at –20°C to –70°C. Reconstituted sTIE-2-His should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
Bioactivity	Biological: Measured in a functional ELISA assay. When TIE-2-His is immobilized at 4μg/ml (100 μl/well), it binds recombinant human Angiopoietin-2 with a linear range of 2-100 ng/ml.
Endotoxin	< 0.1ng per μg sTIE-2-His
Reference Data
RefSeq	NP_000450
Locus ID	7010
UniProt ID	Q02763
Cytogenetics	9p21.2
Synonyms	TIE2, TIE-2, Angiopoietin-1 receptor, p140 TEK
Summary	This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane

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