Dickkopf-3 (DKK3) Human Protein

Specifications

Product Data
Species	Human
Expression Host	CHO
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) APAPTATSAP VKPGPALSYP QEEATLNEMF REVEELMEDT QHKLRSAVEE MEAEEAAAKA SSEVNLANLP PSYHNETNTD TKVGNNTIHV HREIHKITNN QTGQMVFSET VITSVGDEEG RRSHECIIDE DCGPSMYCQF ASFQYTCQPC RGQRMLCTRD SECCGDQLCV WGHCTKMATR GSNGTICDNQ RDCQPGLCCA FQRGLLFPVC TPLPVEGELC HDPASRLLDL ITWELEPDGA LDRCPCASGL LCQPHSHSLV YVCKPTFVGS RDQDGEILLP REVPDEYEVG SFMEEVRQEL EDLERSLTEE MALREPAAAA AALLGGEEI
Predicted MW	36.3 kDa
Purity	>98% pure by SDS-PAGE and HPLC analyses
Buffer	Presentation State: Purified State: Lyophilized (0.2μ Sterile filtered) purified protein
Preparation	Lyophilized (0.2μ Sterile filtered) purified protein
Protein Description	Recombinant human DKK-3 expressed in CHO cells is a glycoprotein that has a calculated molecular weight of 36.3 kDa and contains 329 amino acid residues. Due to glycosylation, human DKK-3 migrates at an apparent molecular weight of approximately 39-49 kDa by SDS-PAGE analysis under non-reducing conditions.
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 E1 cells. The expected ED50 for this effect is 2.0–4.0 ng/ml.
Reference Data
RefSeq	NP_001018067
Locus ID	27122
UniProt ID	Q9UBP4
Cytogenetics	11p15.3
Synonyms	Dickkopf-related protein 3, Dkk-3, REIC
Summary	This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Secreted Protein

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