HCV Core (2-192) Protein

Specifications

Product Data
Expression Host	E. coli
Concentration	lot specific
Buffer	Presentation State: Purified State: Liquid purified fraction (>95% pure by SDS-PAGE). Buffer System: 8 M urea; 20 mM Tris-HCl, pH 8.0, containing 10 mM beta-mercaptoethanol. No preservatives.
Preparation	Liquid purified fraction (>95% pure by SDS-PAGE).
Applications	Suitable in ELISA, Western blot, Colloidal Gold and Latex Beads.
Protein Description	Hepatitis C Virus (HCV) Core Antigen amino cids 2-192 (recombinant) 22 kDa with Beta-galactosidase (114kDa) fused at the N-terminus. Reacts with human HCV positive serum.
Storage	Store the antigen at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid multiple freeze/thaw cycles.
Stability	Shelf life: six months from despatch.
Reference Data
Synonyms	Hepatitis C Virus core protein
Summary	The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR. Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.
Protein Families	Suitable in ELISA, Western blot, Colloidal Gold and Latex Beads.

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