BMP2 / BMP2A Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Predicted MW	26 kDa
Purity	>95% pure by SDS-PAGE and visualised by silver stain
Buffer	Presentation State: Purified State: Lyophilized purified Ig fraction Buffer System: 50 mM Acetic Acid Stabilizer: None
Preparation	Lyophilized purified Ig fraction
Protein Description	Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homodimeric protein. BMP-2 lacks the natural N-terminus which results in a 15-20 fold increase of specific activity. The amino acid sequence of recombinant Human BMP-2 starts with MQAKHKQ (position 283) containing the Met from the E. coli expression vector.
Storage	Lyophilized samples are stable for 6 months from despatch at -20°C to -70°C. Reconstituted BMP-2 should be stored in working aliquots at -20°C. Avoid repeated freezing and thawing.
Bioactivity	Biological: Measured by the ability of BMP-2 to induce Alkaline Phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 0.3-0.8 µg/ml. Specific: 2.5 x 103 units/mg
Endotoxin	< 0.1 ng per µg of BMP-2
Reference Data
RefSeq	NP_001191
Locus ID	650
UniProt ID	P12643
Cytogenetics	20p12.3
Synonyms	Bone morphogenetic protein 2, BMP-2, BMP-2A
Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. [provided by RefSeq, Jul 2016]
Protein Families	Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transmembrane
Protein Pathways	Acute myeloid leukemia, Basal cell carcinoma, Cytokine-cytokine receptor interaction, Endocytosis, Hedgehog signaling pathway, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer, TGF-beta signaling pathway

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