CD332 / FGFR-2 (IIIC - Fc Chimera) Human Protein

Specifications

Product Data
Species	Human
Expression Host	Insect
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) RPSFSLVEDT TLEPEEPPTK YQISQPEVYV AAPGESLEVR CLLKDAAVIS WTKDGVHLGP NNRTVLIGEY LQIKGATPRD SGLYACTASR TVDSETWYFM VNVTDAISSG DDEDDTDGAE DFVSENSNNK RAPYWTNTEK MEKRLHAVPA ANTVKFRCPA GGNPMPTMRW LKNGKEFKQE HRIGGYKVRN QHWSLIMESV VPSDKGNYTC VVENEYGSIN HTYHLDVVER SPHRPILQAG LPANASTVVG GDVEFVCKVY SDAQPHIQWI KHVEKNGSKY GPDGLPYLKV LKAAGVNTTD KEIEVLYIRN VTFEDAGEYT CLAGNSIGIS FHSAWLTVLP APGREKEITA SPDYLEDPRR ASIEGRGDPE EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK
Predicted MW	170 kDa
Purity	>90% by SDS-PAGE and visualised by silver stain.
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: PBS without stabilizers
Preparation	Lyophilized purified protein
Protein Description	Recombinant Human soluble FGFR-2 alpha (IIIc) was fused via a Xa cleavage site with the Fc part of Human IgG1. Human recombinant soluble FGFR-2 alpha (IIIc) is a disulfide-linked heterodimeric protein. In the reduced form the glycosylated subunits of sFGFR-2 alpha/human Fc chimera display a molecular mass of 80-85 kDa.
Note	Centrifuge vials before opening!
Storage	Store lyophilized sFGFR-2a (IIIc)/Fc at -20°C to -70°C. Reconstituted sFGFR-2a (IIIc)/Fc should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles!
Stability	Shelf life: One year from despatch.
Bioactivity	Biological: Determined by its ability to inhibit human FGF acidic-dependent proliferation on R1 cells. The ED50 for this effect is typically at 15.0-30.0 ng/ml.
Endotoxin	< 0.1 ng per µg of sFGF-R2a.
Reference Data
RefSeq	NP_000132
Locus ID	2263
UniProt ID	P21802
Cytogenetics	10q26.13
Synonyms	FGFR2, BEK, KGFR, KSAM
Summary	The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]
Protein Families	Druggable Genome, Protein Kinase, Secreted Protein, Transmembrane
Protein Pathways	Endocytosis, MAPK signaling pathway, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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