CD202b / TEK (Fc Chimera) Human Protein

Specifications

Product Data
Species	Human
Expression Host	Insect
Predicted MW	250 kDa
Purity	>90% by SDS-PAGE and visualised by silver stain
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: PBS without stabilizers
Preparation	Lyophilized purified protein
Protein Description	Recombinant Human soluble TIE-2/Tek was fused with the Fc part of Human IgG1. The recombinant mature sTIE-2/Fc is a disulfide-linked homodimeric protein. The sTIE-2/Fc monomers have a mass of approximately 125 kDa. The soluble receptor protein consists of the full extracellular domain (Met1-Val730). Result by N-terminal sequencing: AMDLILINSL
Note	Centrifuge vials before opening!
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Measured in a functional ELISA assay. When TIE-2/Fc is immobilized at 4 μg/mL (100 μl/well), it binds rh Angiopoietin-2 with a linear range of 2-100 ng/ml.
Endotoxin	< 0.1 ng per µg sTIE-2/Fc.
Reference Data
RefSeq	NP_000450
Locus ID	7010
UniProt ID	Q02763
Cytogenetics	9p21.2
Synonyms	TIE2, TIE-2, Angiopoietin-1 receptor, p140 TEK
Summary	This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane

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