Flt3 ligand Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGG LWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP P SCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSS TLP PPWSPRPLEA TAPTA
Predicted MW	17.6 kDa
Purity	>98% by RP-HPLC, Anion-exchange FPLC, Silverstain
Buffer	Presentation State: Purified State: Lyophilized (freeze-dried) without stabilizer
Preparation	Lyophilized (freeze-dried) without stabilizer
Protein Description	Flt3-ligand produced in E. coli is a soluble non-glycosylated 17.6 kDa polypeptide containing 155 amino acid residues which comprises the extracellular domain of the transmembrane flt3-ligand protein.
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Human rh Flt3-ligand is fully biologically active when compared to standards. The ED50 range is 0.5-1.0 ng/ml corresponding to a specific activity of 1-2MUnits/mg, calculated by the dose-dependant stimulation of the proliferation of Human OCMI-AML5 cells.
Endotoxin	< 0.1 ng per µg (IEU/µg) of rh Flt3-Ligand
Reference Data
RefSeq	NP_001191431
Locus ID	2323
UniProt ID	P49771
Cytogenetics	19q13.33
Synonyms	SL cytokine, FLT3LG, Flt3L
Summary	Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways	Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer

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