Interleukin-6 / IL6 Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Predicted MW	21 kDa
Purity	>98% pure by RP-HPLC, Anion-exchange FPLC, Silverstain.
Buffer	Presentation State: Purified State: Lyophilized purified protein. Buffer System: PBS Stabilizer: None
Preparation	Lyophilized purified protein.
Protein Description	Recombinant human IL-6 produced in E. coli is a single, non-glycosylated polypeptide containing 184 amino acid residues.
Storage	The lyophilized IL-6, although stable at room temperature for 3 weeks, is best stored desiccated at -20°C. Reconstituted Il-6 should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: The ED50 as determined by dose-dependent stimulation of murine hybridoma B9 cells is in the range of 2-10 pg/ml.
Endotoxin	< 0.1 ng per µg (IEU/µg) of rh IL-6
Reference Data
RefSeq	NP_000591
Locus ID	3569
UniProt ID	P05231
Cytogenetics	7p15.3
Synonyms	IL-6, Interferon beta-2, IFNB2, B-cell stimulatory factor 2, BSF-2, CDF
Summary	This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). [provided by RefSeq, Aug 2020]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Jak-STAT signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prion diseases, Toll-like receptor signaling pathway

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