Interleukin-1 beta / IL-1B Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQANMP VFLGGTKGGQ DITDFTMQFV SS
Predicted MW	17 kDa
Purity	>98% pure by SDS-PAGE and Silver stain
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: PBS Stabilizer: None
Preparation	Lyophilized purified protein
Protein Description	Recombinant Human Interleukin-1beta produced in E.Coli is a non-glycosylated, Interleukin-1 beta Polypeptide chain containing 153 amino acids and having a molecular mass of 17.0 kDa. Result by N-terminal sequencing: APVRSL and MAPVRS
Note	Range: 0.1-10.0 ng/ml
Storage	Store lyophilized at RT for 3 weeks or (preferably in a desiccator) at -20°C for longer. Following reconstitution store the antibody undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: Measured in a cell proliferation assay using murine D10G4.1 cells. The ED50 for this effect is typically 2-10 pg/ml.
Endotoxin	< 0.1 ng per µg (IEU/µg) of rh IL-1beta
Reference Data
RefSeq	NP_000567
Locus ID	3553
UniProt ID	P01584
Cytogenetics	2q14.1
Synonyms	IL-1 beta, IL1B, IL1 beta, IL1F2, Catabolin
Summary	The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2020]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Alzheimer's disease, Apoptosis, Cytokine-cytokine receptor interaction, Cytosolic DNA-sensing pathway, Graft-versus-host disease, Hematopoietic cell lineage, MAPK signaling pathway, NOD-like receptor signaling pathway, Prion diseases, Toll-like receptor signaling pathway, Type I diabetes mellitus

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