Adiponectin (biologically active) Human Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDD DDK Glu 1 to Gln 5 were confirmed by N-terminal sequencing.
Concentration	lot specific
Purity	>98%
Buffer	Presentation State: Purified State: Lyophilized purified Protein Buffer System: 20 mM Tris buffer, 20 mM NaCl, pH 7.5
Preparation	Lyophilized purified Protein
Applications	Cell culture and/or animal studies. ELISA. Western Blot.
Protein Description	Human Adiponectin Total 226 AA, UniProt Q15848 (Glu19 – Asn244)
Note	Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage	Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Biological: In vitro gluconeogenesis assay in primary hepatocytes was performed, showing the human adiponectin derived from mammalian cells can inhibit glucose production. The ED50 was ~6 µg/ml.
Reference Data
RefSeq	NP_001171271
Locus ID	9370
UniProt ID	Q15848
Cytogenetics	3q27.3
Synonyms	ADIPOQ, ACDC, ACRP30, APM1, GBP28
Summary	This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
Protein Families	Cell culture and/or animal studies. ELISA. Western Blot.
Protein Pathways	Adipocytokine signaling pathway, PPAR signaling pathway, Type II diabetes mellitus

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