Guanylin Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) GPVTVQDGNF SFSLESVKKL KDLQEPQEPR VGKLRNFAPI PGEPVVPILC SNPNFPEELK PLCKEPNAQE ILQRLEEIAE DPGTCEICAY AACTGC
Concentration	lot specific
Purity	>95%
Buffer	Presentation State: Purified State: Lyophilized purified protein Buffer System: Deionized H2O
Preparation	Lyophilized purified protein
Applications	Western Blot. Protocol: 1. Lauber T, Nourse A, Schulz A, Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12. 2. Rudolph JA, Hawkins JA, Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702. 3. Schulz A, Marx UC, Hidaka Y, Shimonishi Y, Rosch P, Forssmann WG, Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9. 4. Lauber T, Neudecker P, Rosch P, Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.
Protein Description	Total 98 AA. MW: 11 kDa (calculated). 96 AA of the recombinant human Proguanylin and two N-Terminal extra AA (highlighted).
Note	Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage	Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after two weeks at 4°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_291031
Locus ID	2980
UniProt ID	Q02747
Cytogenetics	1p34.2
Synonyms	Guanylate cyclase activator 2A, GUCA2A, GUCA2
Summary	Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins.[UniProtKB/Swiss-Prot Function]
Protein Families	Western Blot. Protocol: 1. Lauber T, Nourse A, Schulz A, Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12. 2. Rudolph JA, Hawkins JA, Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702. 3. Schulz A, Marx UC, Hidaka Y, Shimonishi Y, Rosch P, Forssmann WG, Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9. 4. Lauber T, Neudecker P, Rosch P, Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

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