Creatine kinase M type (CKM) (NM_001824) Human Recombinant Protein
CAT#: TP302721
Recombinant protein of human creatine kinase, muscle (CKM), 20 µg
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Specifications
Product Data | |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>RC202721 protein sequence
Red=Cloning site Green=Tags(s) MPFGNTHNKFKLNYKPEEEYPDLSKHNNHMAKVLTLELYKKLRDKETPSGFTVDDVIQTGVDNPGHPFIM TVGCVAGDEESYEVFKELFDPIISDRHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGY TLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEKEQQQLIDDHFLFDKPVSPLLLASGMARD WPDARGIWHNDNKSLLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNQHLGYV LTCPSNLGTGLRGGVHVKLAHLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQV QLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-Myc/DDK |
Predicted MW | 42.9 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_001815 |
Locus ID | 1158 |
UniProt ID | P06732 |
Refseq Size | 1666 |
Cytogenetics | 19q13.32 |
Refseq ORF | 1143 |
Synonyms | CKMM; CPK-M; M-CK |
Summary | The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008] |
Protein Families | Druggable Genome |
Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
Citations (1)
The use of this Proteins has been cited in the following citations: |
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Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle
,null,
PLoS ONE
,PubMed ID 30395621
[CKM]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC400690 | CKM HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
LY400690 | Transient overexpression lysate of creatine kinase, muscle (CKM) |
CNY 3,080.00 |
|
PH302721 | CKM MS Standard C13 and N15-labeled recombinant protein (NP_001815) |
CNY 19,520.00 |
|
TP721121 | Purified recombinant protein of Human creatine kinase, muscle (CKM) |
CNY 3,140.00 |
|
TP750145 | Purified recombinant heterodimer protein of Human CKMB(full length creatine kinase, muscle (CKM) with N-terminal GST tag, and full length creatine kinase, brain (CKB), with N-terminal His(CKB) tag), expressed in E.coli, 100ug |
CNY 2,841.00 |
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