Epac2 (RAPGEF4) (NM_007023) Human Recombinant Protein
CAT#: TP305335
Recombinant protein of human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1, 20 µg
View other "Epac2" proteins (3)
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Specifications
Product Data | |
Species | Human |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>RC205335 protein sequence
Red=Cloning site Green=Tags(s) MVAAHAAHSSSSAEWIACLDKRPLERSSEDVDIIFTRLKEVKAFEKFHPNLLHQICLCGYYENLEKGITL FRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIGTAFGESILDNTPRHATIVTRESSELLRIEQ KDFKALWEKYRQYMAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITKVPSEKILRAGKIL RNAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQEHHF QDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEE LLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDD FGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQG NSQPQQKYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQ GTEQEKMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKEQLPELEKIV KQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCMDPTYTTIRVPVATSVKEVISAVA DKLGSGEGLIIVKMSSGGEKVVLKPNDVSVFMTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFEL MSSKDLAYQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKR VQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYR LTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNH QDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-Myc/DDK |
Predicted MW | 115.3 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_008954 |
Locus ID | 11069 |
UniProt ID | Q8WZA2 |
Refseq Size | 4361 |
Cytogenetics | 2q31.1 |
Refseq ORF | 3033 |
Synonyms | CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496 |
Summary | Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Protein Pathways | Leukocyte transendothelial migration |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC416236 | RAPGEF4 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
LY416236 | Transient overexpression lysate of Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 1 |
CNY 3,080.00 |
|
PH305335 | RAPGEF4 MS Standard C13 and N15-labeled recombinant protein (NP_008954) |
CNY 19,520.00 |