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Isocitrate dehydrogenase (IDH1) (NM_005896) Human Recombinant Protein
CAT＃: TP310582
Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), 20 µg
View other "Isocitrate dehydrogenase" proteins (19)
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| Cited in 3 publications. |
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Specifications
| Product Data | |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence |
>RC210582 representing NM_005896
Red=Cloning site Green=Tags(s) MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVG VKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYR ATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLS TKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDS VAQGYGSLGMMTSVLVCPDGKTVEAESAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNK ELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Tag | C-Myc/DDK |
| Predicted MW | 46.5 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Bioactivity | Enzymatic activities were determined by monitoring NADPH formation based on the absorbance at 345nm. The reaction was carried out at 37? for 10 minutes in the presence of isocitrate as a substrate and NADP as a cofactor. The data which presented a good linear relation on the curve was used to calculate the specific activity, and one unit is defined as converting 1.0 umole of NADP to NADPH per min at 37?. In summary, the wildtype IDH1 produced from HEK293 cells and insect cells are active while the R132H mutant or the WT/R132H heterodimers are inactive. |
| Reference Data | |
| RefSeq | NP_005887 |
| Locus ID | 3417 |
| UniProt ID | O75874 |
| Refseq Size | 2339 |
| Cytogenetics | 2q34 |
| Refseq ORF | 1242 |
| Synonyms | HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD |
| Summary | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] |
| Protein Pathways | Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways |
Citations (3)
| The use of this Proteins has been cited in the following citations: |
|---|
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Antibody Validation for Western blot: By the User, for the User
,Pillai-Kastoori, L;Heaton, S;Shiflett, SD;Roberts, AC;Solache, A;Schutz-Geschwender, AR;,
J. Biol. Chem.
,PubMed ID 31819006
[IDH1]
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G6pd Deficiency Does Not Affect the Cytosolic Glutathione or Thioredoxin Antioxidant Defense in Mouse Cochlea
,null,
The Journal of Neuroscience
,PubMed ID 28473643
[IDH1]
|
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A Highly Facile and Specific Assay for Cancer-Causing Isocitrate Dehydrogenase Mutant Using (13)C4-Labeled a-Ketoglutarate and Heteronuclear NMR
,Wen, H;Yun, T;Xu, WJ;Choi, SH;Kim, H;Park, CK;Lee, SH;Park, SW;Lee, SK;Park, S;,
Anal. Chem. Dec 2013.
,PubMed ID 24266712
[IDH1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC401782 | IDH1 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
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| LY401782 | Transient overexpression lysate of isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
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| PH310582 | IDH1 MS Standard C13 and N15-labeled recombinant protein (NP_005887) |
CNY 19,520.00 |
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| TP700038 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132H),with C-terminal Myc-DDK tag, expressed in HEK293 cells |
CNY 6,650.00 |
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| TP700039 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132C),with C-terminal Myc-DDK tag, expressed in HEK293 cells |
CNY 6,650.00 |
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| TP700040 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132L),with C-terminal Myc-DDK tag, expressed in HEK293 cells |
CNY 6,650.00 |
|
| TP700041 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132S),with C-terminal Myc-DDK tag, expressed in HEK293 cells |
CNY 6,650.00 |
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| TP700042 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132G),with C-terminal Myc-DDK tag, expressed in HEK293 cells |
CNY 6,650.00 |
|
| TP710042 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), with C-terminal polyhistidine tag, expressed in sf9 cells. |
CNY 4,020.00 |
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| TP710043 | Recombinant protein of mutant(R132H) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. |
CNY 4,020.00 |
|
| TP710044 | Recombinant protein of mutant(R132C) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells |
CNY 4,020.00 |
|
| TP710045 | Recombinant protein of mutant(R132L) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. |
CNY 4,020.00 |
|
| TP710046 | Recombinant protein of mutant(R132S) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. |
CNY 4,020.00 |
|
| TP710047 | Recombinant protein of mutant(R132G) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. |
CNY 4,020.00 |
|
| TP710048 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132H) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells. |
CNY 4,020.00 |
|
| TP710049 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132C) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
CNY 4,020.00 |
|
| TP710050 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132L) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
CNY 4,020.00 |
|
| TP710051 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132S) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
CNY 4,020.00 |
|
| TP710052 | Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132G) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells |
CNY 4,020.00 |
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