Dhps (BC039963) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR201742 protein sequence Red=Cloning site Green=Tags(s) MPILDQMVLEQNTEGVKWTPSKMISRLGKEINNPDSVYYWAHKNHIPVLSPALTDGSLGDMIFFHSYKNP GLVLDIVEDLRLINTQAIFAKRSGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDE AVSWGKIRMDAQPVKVYADASLVFPLLVAETFAQKADAFRAEKNED TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	20.6 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
Locus ID	330817
UniProt ID	Q3TXU5
Refseq Size	1149
Cytogenetics	8 C3
Refseq ORF	558
Synonyms	Dhs, MGC49129, MGC74384
Summary	Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.[UniProtKB/Swiss-Prot Function]

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