Sh3bp1 (BC004598) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR209303 protein sequence Red=Cloning site Green=Tags(s) MAESFKELDPDSSMGKALEMTCAIQNQLARILAEFEMTLERDVLQPLSRLSEEELPAILKHKKSLQKLVS DWNTLKSRLSQAAKNSGSNQGLGGASGSHTHTTTANKVEMLKEEEEELKKKVEQCKDEYLADLYHFSTKE DSYANYFIHLLEIQADYHRKSLTSLDTALAELRDNHNQADHSPLTTAAPFSRVYGVSLRTHLQDLGRDIA LPIEACVLLLLSEGMQEEGLFRLAAGASVLKRLKQTMASDPHSLEEFCSDPHAVAGALKSYLRELPEPLM TSDLYDDWMRAASLKEPGARLEALHDVCSRLPQENFNNLRYLMKFLALLAEEQDVNKMTPSNIAIVLGPN LLWPPEKEGDQAQLDAASVSSIQVVGVVEALIQNADTLFPGDINFNVSGIFPGLAPQEKVSSQQVSEELP PVTVPAPATTPAPTLAPASMAVRERTEADLPKPTSPKVSRNPTETAASAEDMTRKTKRPAPARPTMPPPQ PSSTRSSPPAPSLPPGSVSPGTPQALPRRLVGTSLRAPTMPPPLPPVPPQPARRQSRRLPASPVISNMPA QVDQGVATEDREGPEAVGGHPPPPALPPQPRPRGLISETE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	65.3 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
Locus ID	20401
Refseq Size	2224
Cytogenetics	15 E1
Refseq ORF	1800
Synonyms	3BP-1
Summary	GTPase activating protein (GAP) which specifically converts GTP-bound Rho-type GTPases including RAC1 and CDC42 in their inactive GDP-bound form (PubMed:7621827). By specifically inactivating RAC1 at the leading edge of migrating cells, it regulates the spatiotemporal organization of cell protrusions which is important for proper cell migration. Also negatively regulates CDC42 in the process of actin remodeling and the formation of epithelial cell junctions. Through its GAP activity toward RAC1 and/or CDC42 plays a specific role in phagocytosis of large particles. Specifically recruited by a PI3 kinase/PI3K-dependent mechanism to sites of large particles engagement, inactivates RAC1 and/or CDC42 allowing the reorganization of the underlying actin cytoskeleton required for engulfment. It also plays a role in angiogenesis and the process of repulsive guidance as part of a semaphorin-plexin signaling pathway. Following the binding of PLXND1 to extracellular SEMA3E it dissociates from PLXND1 and inactivates RAC1, inducing the intracellular reorganization of the actin cytoskeleton and the collapse of cells (By similarity).[UniProtKB/Swiss-Prot Function]

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