Gfm1 (NM_138591) Mouse Recombinant Protein

Specifications

Product Data
Species	Mouse
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) >MR210468 protein sequence Red=Cloning site Green=Tags(s) MRLLRVAAALGRGPFPRVPAVLGWQGKQADWKTRRWCSSGPVPNEKIRNIGISAHIDSGKTTLTERVLYY TGRIATMHEVKGKDGVGAVMDSMELERQRGITIQSAATYTMWKDININIIDTPGHVDFTIEVERALRVLD GAVLVLCAVGGVQCQTMTVSRQMKRYNVPFLTFINKLDRMGSNPSRALQQMRSKLNHNAAFVQIPIGLEG DFKGIIDLIEERAIYFDGDFGQIVRYDEIPAGLRAAAADHRQELIECVANSDEQLGELFLEEKIPSVSDL KRAIRRATLSRSFTPVFLGSALKNKGVQPLLDAVLEYLPNPSEVQNYAILNQNDSKEKTKILMNPQRDDS HPFVGLAFKLEAGRFGQLTYVRNYQGELKKGSTIYNTRTGKKVRVQRLVRMHADMMEDVEEVYAGDICAL FGIDCASGDTFTNKDNSDLSMESIHVPEPVISIAMRPSNKNDLEKFSKGIGRFTREDPTFKVHFDPESKE TIVSGMGELHLEIYAQRMEREYGCSCITGKPKVAFRETIVAPVPFDFTHKKQSGGAGQFGKVIGVLEPLP PEDYTKLEFSDETFGSNVPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAG EGALKQALANGTLCIIEPIMSVEVIAPNEFQGTVFAGINRRHGVITGQDGIEDYFTLYADVPLNNMFGYS TELRSCTEGKGEYTMEYCRYQPCSPSTQEELINKYLEATGQLPVKKGKAKN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag	C-MYC/DDK
Predicted MW	83.5 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer	25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_613057
Locus ID	28030
UniProt ID	Q8K0D5
Refseq Size	2598
Cytogenetics	3 30.96 cM
Refseq ORF	2256
Synonyms	AW545374; D3Wsu133e; Gfm
Summary	Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.[UniProtKB/Swiss-Prot Function]

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