Tut1 (NM_197993) Mouse Recombinant Protein
CAT#: TP511013
Purified recombinant protein of Mouse terminal uridylyl transferase 1, U6 snRNA-specific (Tut1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
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CNY 7,106.00
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CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Species | Mouse |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence |
>MR211013 protein sequence
Red=Cloning site Green=Tags(s) MAAVDSDVVSLPRGRFRCCLCDVTTANRPSLDAHLKGRKHRDLVQLRATRKAQGLRSVFVSGFPRDVGSA QLSEYFQTFGPVANIVMDKDKGVFAIVEMGDISAREAVLSQPKHSLGGHGLRVRPREQKEFQSPASKSPK GVDSSSHQLVQALAEAADVGAQMVKLVELRELSEAERQLRNLVVALMQEVFTEFFPGCVVHPFGSTVNSF DVHGCDLDLFLDMGDMEETEPDPKAPKVPETSSLDSALASSLDPQALACTPASPLDSLSPTSVQESESLD FDTPSSLAPQTPDSALGSDTVTSPQSLPPVSPLQEDRKEGKQGKELELAEEASKDEKEEAAAVLELVGSI LRGCVPGVYRVQTVPSARRPVVKFCHRPSGLHGDVSLSNRLALYNSRFLNLCSEMDGRVRPLVYTLRCWA QHNGLSGGGPLLNNYALTLLVIYFLQTRDPPVLPTVAQLTQRAGEGEQVEVDGWDCSFPKDASRLEPSTN VEPLSSLLAQFFSCVSCLDLSGSLLSLREGRPLMVAEGLPSDLWEGLRLGPMNLQDPFDLSHNVAANVTG RVAKRLQSCCGAAASYCRSLQYQQRSSRGRDWGLLPLLQPSSPSSLLSAKLIPLPSAPFPQVIMALVDVL REALGCHIEQGTKRRRSEGARIKDSPLGGVNKRQRLGGQEKSFEEGKEEPQGCAGDHSENEVEEMVIEVR ETPQDWALLHSGPPEEELPLMTANCLDKAAEHNPMKPEVAGEGSQGETGKEASHPSSVSWRCALWHQVWQ GRRRARRRLQQQTKEEGRGGPTTGAEWLAMEARVTQELKGPNSEQERPPGEPLLSFVASASQAEQTLTVA PLQDSQGLFPGLHHFLQGFIPQALKNLLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Tag | C-MYC/DDK |
Predicted MW | 94.6 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C after receiving vials. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_932110 |
Locus ID | 70044 |
UniProt ID | Q8R3F9 |
Refseq Size | 2764 |
Cytogenetics | 19 A |
Refseq ORF | 2610 |
Synonyms | 2700038E08Rik; PAPD2; Rbm21; star-PAP; TUTase6; U6-TUTase |
Summary | Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3' UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3' UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
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