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B Raf (BRAF) (NM_004333) Mutant (V600E) Human Recombinant Protein
CAT＃: TP700033
Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) kinase domain V600E mutant, expressed in human cells
View other "B Raf" proteins (13)
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CNY 6,650.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence |
A DNA sequence from TrueORF clone, RC211013, encoding the Vpolyhistidine766) of human BRAF
|
| Tag | C-DDK |
| Predicted MW | 44 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Bioactivity | BRAF kinase activity was measured in an HTRF® assay. Varying concentrations of BRAF were added to a reaction mix containing ATP and a biotinylated kinase substrate (HTRF substrate 2) and was incubated at 37C for phosphorylation. HTRF detection reagents were then added, the reaction was incubated for 30 minutes at room temperature. Time-resolved fluorescent signal (Delta R) was measured on a Flexstation 3 microplate reader. |
| Reference Data | |
| RefSeq | NP_004324 |
| Locus ID | 673 |
| UniProt ID | P15056 |
| Refseq Size | 2949 |
| Cytogenetics | 7q34 |
| Refseq ORF | 2298 |
| Synonyms | B-raf; B-RAF1; BRAF1; NS7; RAFB1 |
| Summary | This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017] |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Acute myeloid leukemia, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Glioma, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Thyroid cancer, Vascular smooth muscle contraction |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC401382 | BRAF HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY401382 | Transient overexpression lysate of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) |
CNY 3,080.00 |
|
| PH311013 | BRAF MS Standard C13 and N15-labeled recombinant protein (NP_004324) |
CNY 19,520.00 |
|
| TP311013 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), 20 µg |
CNY 2,900.00 |
|
| TP700031 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600E mutant, expressed in human cells |
CNY 6,650.00 |
|
| TP700032 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) kinase domain, expressed in human cells |
CNY 6,650.00 |
|
| TP700044 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600A mutant, expressed in human cells |
CNY 6,650.00 |
|
| TP700046 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600G mutant, expressed in human cells |
CNY 6,650.00 |
|
| TP700047 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600M mutant, expressed in human cells |
CNY 6,650.00 |
|
| TP700048 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600D mutant, expressed in human cells |
CNY 6,650.00 |
|
| TP700049 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600R mutant, expressed in human cells |
CNY 6,650.00 |
|
| TP700050 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) V600K mutant, expressed in human cells |
CNY 6,650.00 |
|
| TP700051 | Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF) K601E mutant, expressed in human cells |
CNY 6,650.00 |
