IL15 (NM_000585) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human IL-15, the region of Asn49-Ser162, from gene Accession# NM_000585
Tag	Tag Free
Predicted MW	12.8 kDa
Concentration	lot specific
Purity	Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Buffer	10 mM NaH2PO4, 150 mM NaCl, pH 7.2
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 0.10-0.50 ng/ml, corresponding to a specific activity of 2-10 x 10^6 units/mg, determined by the dose dependent stimulation of CTLL-2 cells proliferation.
Endotoxin	Less than 0.01 ng per ug protein as determined by the LAL method
Reference Data
RefSeq	NP_000576
Locus ID	3600
UniProt ID	P40933
Refseq Size	2012
Cytogenetics	4q31.21
Refseq ORF	486
Synonyms	IL-15
Summary	The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

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