IL13 (NM_002188) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human IL-13, the region of Gly21-Asn132, from gene Accession# NM_002188
Tag	Tag Free
Predicted MW	12 kDa
Concentration	lot specific
Purity	Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Buffer	10 mM NaH2PO4, 150 mM NaCl, pH 7.2
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 1.5 to 3ng/ml, corresponding to a specific activity of 6.6 -- 3.3 x 10^5units/mg, determined by the dose dependent stimulation of TF-1 cells proliferation.
Endotoxin	Less than 0.01 ng per µg protein as determined by the LAL method
Reference Data
RefSeq	NP_002179
Locus ID	3596
UniProt ID	P35225
Refseq Size	1282
Cytogenetics	5q31.1
Refseq ORF	438
Synonyms	IL-13; P600
Summary	This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Asthma, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Jak-STAT signaling pathway

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