MCP1 (CCL2) (NM_002982) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human MCP1, the region of Gln24-Thr99, from gene Accession# NM_002982
Tag	Tag Free
Predicted MW	8.7 kDa
Concentration	lot specific
Purity	Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Buffer	10 mM NaHPO4 pH 7.2, 0.15M NaCl
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 6-15 ng/ml, corresponding to a specific activity of 1.6-0.6 x 10^5 units/mg, determined by the dose dependent chemoattraction of THP-1 cells
Endotoxin	Less than 0.01 ng per µg protein as determined by the LAL method
Reference Data
RefSeq	NP_002973
Locus ID	6347
UniProt ID	P13500
Refseq Size	760
Cytogenetics	17q12
Refseq ORF	297
Synonyms	GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
Summary	This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Elevated expression of the encoded protein is associated with severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection. [provided by RefSeq, Aug 2020]
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Chemokine signaling pathway, Cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway

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