Tnf (NM_012675) Rat Recombinant Protein

Specifications

Product Data
Species	Rat
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Rat TNF-a, the region of Leu80-Leu235, from gene Accession# NM_012675
Tag	Tag Free
Predicted MW	The 157 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of approximately 17,361 Da. The DTT-reduced protein migrates at approximately 17kDa and the non-reduced protein migrates at approximately 16kDa by SDS-PAGE.
Concentration	lot specific
Purity	>98%, as determined by Coomassie stained SDS-PAGE.
Buffer	10 mM NaH2PO4, 150 mM NaCl, pH 7.2
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Endotoxin	Less than 0.1 EU/µg (<0.01 ng/µg) protein as determined by the LAL method
Reference Data
RefSeq	NP_036807
Locus ID	24835
UniProt ID	P16599
Refseq Size	1687
Cytogenetics	20p12
Refseq ORF	705
Synonyms	RATTNF; TNF-alpha; Tnfa
Summary	acts as a cytokine; binds TNF receptors; plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response [RGD, Feb 2006]

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