TSLP (NM_033035) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human TSLP,the rehion of Met- Tyr29-Gln159, from gene accession# NM_033035.4
Tag	Tag Free
Predicted MW	15 kDa
Concentration	lot specific
Purity	>98%, as determined by Coomassie stained SDS-PAGE.
Buffer	20 mM NaHPO4, pH 7.2, 0.15 M NaCl
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 1 - 5 ng/ml, corresponding to a specific activity of 0.2 - 1 x 10^6 units/mg, determined by the dose dependent stimulation of mouse BaF3-human IL-7a and human TSLPR cotransfectant cell proliferation.
Endotoxin	Less than 0.01 ng per µg protein as determined by the LAL method
Reference Data
RefSeq	NP_149024
Locus ID	85480
UniProt ID	Q969D9
Refseq Size	2652
Cytogenetics	5q22.1
Refseq ORF	477
Summary	This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. In addition, the shorter (predominant) isoform is an antimicrobial protein, displaying antibacterial and antifungal activity against B. cereus, E. coli, E. faecalis, S. mitis, S. epidermidis, and C. albicans. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2020]
Protein Families	Druggable Genome
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

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