TNFRSF1B (NM_001066) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human TNF-RII, the region of Pro 24-Thr206 with an N-terminal Met, from gene Accession# AAA36755
Tag	Tag Free
Predicted MW	20 kDa
Concentration	lot specific
Purity	>95%, as determined by Coomassie stained SDS-PAGE
Buffer	1 x PBS
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 0.15-0.5 µg/ml, corresponding to a specific activity of 2-6 x 103 units/mg, determined by a dose-dependent inhibition of 0.25 ng/ml TNF-a induced cytotoxicity in L929 mouse fibroblast cells in the presence of 4 µg/ml actinomycin D.
Endotoxin	Less than 0.01 ng per µg protein as determined by the LAL method
Reference Data
RefSeq	NP_001057
Locus ID	7133
UniProt ID	P20333
Refseq Size	3682
Cytogenetics	1p36.22
Refseq ORF	1383
Synonyms	CD120b; p75; p75TNFR; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80
Summary	The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome, Secreted Protein, Transmembrane
Protein Pathways	Adipocytokine signaling pathway, Amyotrophic lateral sclerosis (ALS), Cytokine-cytokine receptor interaction

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