PCSK9 (NM_174936) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	CHO
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human PCSK9, the region of Arg29-Gln692, from gene Accession# NM_174936
Tag	C-His
Predicted MW	73 kDa
Concentration	lot specific
Purity	>90%, as determined by Coomassie stained SDS-PAGE.
Buffer	25 mM sodium Acetate, 150 mM NaCl, pH 5.0
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	When recombinant human LDLR is immobilized at 1 µg/mL, recombinant human PCSK9 binds with EC50 of 0.04 - 0.2 µg/mL in a functional ELISA.
Endotoxin	Less than 1 EU per µg protein as determine by the LAL method
Reference Data
RefSeq	NP_777596
Locus ID	255738
UniProt ID	Q8NBP7
Refseq Size	3636
Cytogenetics	1p32.3
Refseq ORF	2076
Synonyms	FH3; FHCL3; HCHOLA3; LDLCQ1; NARC-1; NARC1; PC9
Summary	This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Protein Families	Secreted Protein

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