LIF (NM_002309) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) Human LIF, the region of Ser23-Phe202, from gene Accession# NM_002309
Tag	Tag Free
Predicted MW	20 kDa
Concentration	lot specific
Purity	>97%, as determined by Coomassie stained SDS-PAGE.
Buffer	1 x PBS, pH 7.4
Storage	Store at -80°C.
Stability	Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Bioactivity	The ED50 is 0.03-0.12 ng/ml, corresponding to a specific activity of 8.3-33.3 x 106 units/mg.
Endotoxin	Less than 1.0 EU per µg of protein as determine by the LAL method
Reference Data
RefSeq	NP_002300
Locus ID	3976
UniProt ID	P15018
Refseq Size	3987
Cytogenetics	22q12.2
Refseq ORF	606
Synonyms	CDF; DIA; HILDA; MLPLI
Summary	The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]
Protein Families	Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

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