IDE (42-1019, His-Tag) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	Baculovirus
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MNNPAIKRIG NHITKSPEDK REYRGLELAN GIKVLLISDP TTDKSSAALD VHIGSLSDPP NIAGLSHFCE HMLFLGTKKY PKENEYSQFL SEHAGSSNAF TSGEHTNYYF DVSHEHLEGA LDRFAQFFLC PLFDESCKDR EVNAVDSEHE KNVMNDAWRL FQLEKATGNP KHPFSKFGTG NKYTLETRPN QEGIDVRQEL LKFHSAYYSS NLMAVCVLGR ESLDDLTNLV VKLFSEVENK NVPLPEFPEH PFQEEHLKQL YKIVPIKDIR NLYVTFPIPD LQKYYKSNPG HYLGHLIGHE GPGSLLSELK SKGWVNTLVG GQKEGARGFM FFIINVDLTE EGLLHVEDII LHMFQYIQKL RAEGPQEWVF QECKDLNAVA FRFKDKERPR GYTSKIAGIL HYYPLEEVLT AEYLLEEFRP DLIEMVLDKL RPENVRVAIV SKSFEGKTDR TEEWYGTQYK QEAIPDEVIK KWQNADLNGK FKLPTKNEFI PTNFEILPLE KEATPYPALI KDTAMSKLWF KQDDKFFLPK ACLNFEFFSP FAYVDPLHCN MAYLYLELLK DSLNEYAYAA ELAGLSYDLQ NTIYGMYLSV KGYNDKQPIL LKKIIEKMAT FEIDEKRFEI IKEAYMRSLN NFRAEQPHQH AMYYLRLLMT EVAWTKDELK EALDDVTLPR LKAFIPQLLS RLHIEALLHG NITKQAALGI MQMVEDTLIE HAHTKPLLPS QLVRYREVQL PDRGWFVYQQ RNEVHNNCGI EIYYQTDMQS TSENMFLELF CQIISEPCFN TLRTKEQLGY IVFSGPRRAN GIQGLRFIIQ SEKPPHYLES RVEAFLITME KSIEDMTEEA FQKHIQALAI RRLDKPKKLS AECAKYWGEI ISQQYNFDRD NTEVAYLKTL TKEDIIKFYK EMLAVDAPRR HKVSVHVLAR EMDSCPVVGE FPCQNDINLS QAPALPQPEV IQNMTEFKRG LPLFPLVKPH INFMAAKL
Tag	His-Tag
Predicted MW	114kDa (984aa)
Concentration	0.5mg/ml (determined by Absorbance at 280nm)
Purity	> 90% by SDS-PAGE
Buffer	Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 0.05% Brij35, 10% glycerol
Applications	SDS-PAGE, Enzyme Activity
Storage	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Bioactivity	Specific activity is > 3,000 pmol/min/ug in which one unit will convert 1.0 pmole of Mca-RPPGFSAFK(Dnp)-OH to MCA-Pro-Leu-OH per minute at pH 7.5 at 25°C.
Endotoxin	< 1 EU per 1ug of protein (determined by LAL method)
Reference Data
RefSeq	NP_004960.2
UniProt ID	P14735
Synonyms	Insulin-degrading enzyme, insulin-degrading enzyme isoform 1, Abeta-degrading protease, Insulin protease, Insulysin, IDE
Summary	Insulysin/IDE, also known insulin-degrading enzyme, is a zinc metallopeptidase of the inverzincin family. This enzyme catalyses the degradation reaction of insulin, glucagon and other polypeptides. It is present in mammals and in many arthropods such as the fly Drosophila melanogaster. It is also expressed in many tissues, with the highest levels in liver, kidney, brain, and testis. It has been shown to degrade the amyloid beta peptide, which polymerizes into the plaques associated with Alzheimers disease. Therefore, the deficiency of IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus and Alzheimers disease. Recombinant human Insulysin/IDE, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Protein Families	SDS-PAGE, Enzyme Activity

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