KGF (FGF7) (NM_002009) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding the region (Cys32-Thr194) of human FGF7
Tag	Tag Free
Predicted MW	19 kDa
Concentration	Resuspend the protein to the desired concentration in proper buffer.
Purity	>95% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Lyophilized from a sterile solution containing 20 mM PB, pH 7.4
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity	Determined by the dose-dependant stimulation of 4MBr-5 cells. ED50 for this effect is typically 0.92-1.38 ng/ml.
Endotoxin	< 0.1 EU per 1 µg of the protein by the LAL
Reference Data
RefSeq	NP_002000
Locus ID	2252
UniProt ID	P21781
Refseq Size	3853
Cytogenetics	15q21.2
Refseq ORF	582
Synonyms	HBGF-7; KGF
Summary	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]
Protein Families	Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

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