FGF10 (NM_004465) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding the region (Leu40-Ser208) of human FGF10
Tag	Tag Free
Predicted MW	19.3 kDa
Concentration	Resuspend the protein to the desired concentration in proper buffer.
Purity	>95% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Lyophilized from a sterile solution containing 20 mM PB, pH 7.4
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity	Determined by the dose-dependant stimulation of 4MBr-5 cells. ED50 for this effect is typically 14 - 21 ng/ml.
Endotoxin	< 0.1 EU per 1 µg of the protein by the LAL
Reference Data
RefSeq	NP_004456
Locus ID	2255
UniProt ID	O15520
Refseq Size	627
Cytogenetics	5p12
Refseq ORF	624
Summary	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008]
Protein Families	Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways	MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

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