Cardiac Troponin I (TNNI3) (NM_000363) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence encoding human full-length TNNI3
Tag	N-His
Predicted MW	23.8 kDa
Concentration	Concentration is determined by spectrophotometrically（A280）
Purity	> 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer	Lyophilized from 0.01 M HCl, reconstitute with Tris/urea buffer (20 mM Tris, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM β-mercaptoethanol)
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -20°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_000354
Locus ID	7137
UniProt ID	P19429
Refseq Size	2073
Cytogenetics	19q13.42
Refseq ORF	630
Synonyms	CMD1FF; CMD2A; CMH7; cTnI; RCM1; TNNC1
Summary	Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19. [provided by RefSeq, Aug 2020]
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency
Protein Pathways	Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)

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