CPSF30 (CPSF4) (NM_001081559) Human Recombinant Protein
CAT#: TP761332
Purified recombinant protein of Human cleavage and polyadenylation specific factor 4, 30kDa (CPSF4), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug
View other "CPSF30" proteins (5)
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CNY 2,040.00
Specifications
Product Data | |
Species | Human |
Expression Host | E. coli |
Expression cDNA Clone or AA Sequence |
A DNA sequence encoding human full-length CPSF4
|
Tag | N-GST and C-His |
Predicted MW | 53.4 kDa |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Buffer | 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol |
Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage | Store at -80°C. |
Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Reference Data | |
RefSeq | NP_001075028 |
Locus ID | 10898 |
UniProt ID | O95639 |
Refseq Size | 1754 |
Cytogenetics | 7q22.1 |
Refseq ORF | 732 |
Synonyms | CPSF30; NAR; NEB-1; NEB1 |
Summary | Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC416456 | CPSF4 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
LC421163 | CPSF4 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
LC429309 | CPSF4 HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
LY416456 | Transient overexpression lysate of cleavage and polyadenylation specific factor 4, 30kDa (CPSF4), transcript variant 1 |
CNY 3,080.00 |
|
LY421163 | Transient overexpression lysate of cleavage and polyadenylation specific factor 4, 30kDa (CPSF4), transcript variant 2 |
CNY 3,080.00 |