Collagen I (COL1A1) (NM_000088) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) A DNA sequence from TrueORF clone, RC206234, encoding the region Gln300-Asp744 of COL1A1
Tag	N-His
Predicted MW	40.8 kDa
Concentration	>0.05 µg/µL as determined by microplate BCA method
Purity	> 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer	PBS, pH 7.4, 10% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -80°C.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data
RefSeq	NP_000079
Locus ID	1277
UniProt ID	P02452
Refseq Size	5927
Cytogenetics	17q21.33
Refseq ORF	4392
Synonyms	CAFYD; EDSARTH1; EDSC; OI1; OI2; OI3; OI4
Summary	This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion

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