LwCas13a Leptotrichia wadei Recombinant Protein

Specifications

Product Data
Species	Leptotrichia wadei
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) 1 MHHHHHHENL YFQGAIAPKK KRKVEDPKKK RKVDMKVTKV DGISHKKYIE 51 EGKLVKSTSE ENRTSERLSE LLSIRLDIYI KNPDNASEEE NRIRRENLKK 101 FFSNKVLHLK DSVLYLKNRK EKNAVQDKNY SEEDISEYDL KNKNSFSVLK 151 KILLNEDVNS EELEIFRKDV EAKLNKINSL KYSFEENKAN YQKINENNVE 201 KVGGKSKRNI IYDYYRESAK RNDYINNVQE AFDKLYKKED IEKLFFLIEN 251 SKKHEKYKIR EYYHKIIGRK NDKENFAKII YEEIQNVNNI KELIEKIPDM 301 SELKKSQVFY KYYLDKEELN DKNIKYAFCH FVEIEMSQLL KNYVYKRLSN 351 ISNDKIKRIF EYQNLKKLIE NKLLNKLDTY VRNCGKYNYY LQVGEIATSD 401 FIARNRQNEA FLRNIIGVSS VAYFSLRNIL ETENENGITG RMRGKTVKNN 451 KGEEKYVSGE VDKIYNENKQ NEVKENLKMF YSYDFNMDNK NEIEDFFANI 501 DEAISSIRHG IVHFNLELEG KDIFAFKNIA PSEISKKMFQ NEINEKKLKL 551 KIFKQLNSAN VFNYYEKDVI IKYLKNTKFN FVNKNIPFVP SFTKLYNKIE 601 DLRNTLKFFW SVPKDKEEKD AQIYLLKNIY YGEFLNKFVK NSKVFFKITN 651 EVIKINKQRN QKTGHYKYQK FENIEKTVPV EYLAIIQSRE MINNQDKEEK 701 NTYIDFIQQI FLKGFIDYLN KNNLKYIESN NNNDNNDIFS KIKIKKDNKE 751 KYDKILKNYE KHNRNKEIPH EINEFVREIK LGKILKYTEN LNMFYLILKL 801 LNHKELTNLK GSLEKYQSAN KEETFSDELE LINLLNLDNN RVTEDFELEA 851 NEIGKFLDFN ENKIKDRKEL KKFDTNKIYF DGENIIKHRA FYNIKKYGML 901 NLLEKIADKA KYKISLKELK EYSNKKNEIE KNYTMQQNLH RKYARPKKDE 951 KFNDEDYKEY EKAIGNIQKY THLKNKVEFN ELNLLQGLLL KILHRLVGYT 1001 SIWERDLRFR LKGEFPENHY IEEIFNFDNS KNVKYKSGQI VEKYINFYKE 1051 LYKDNVEKRS IYSDKKVKKL KQEKKDLYIR NYIAHFNYIP HAEISLLEVL 1101 ENLRKLLSYD RKLKNAIMKS IVDILKEYGF VATFKIGADK KIEIQTLESE 1151 KIVHLKNLKK KKLMTDRNSE ELCELVKVMF EYKALEPKKK RKVEDPKKKR 1201 KVDTR
Tag	N-His
Predicted MW	144.9 kDa
Concentration	10uM
Purity	> 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer	10 mM Tris-HCl, pH 7.4, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol
Note	For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage	Store at -20℃ after receiving vials.
Stability	Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Endotoxin	< 0.5 EU per 1 µg of the protein by the LAL
Reference Data

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