NFAT1 (NFATC2) Human shRNA Plasmid Kit (Locus ID 4773)

Specifications

Product Data
Product Name	NFAT1 (NFATC2) Human shRNA Plasmid Kit (Locus ID 4773)
Locus ID	4773
UniProt ID	Q13469
Synonyms	NFAT1; NFATP
Vector	pRFP-C-RS
Format	Retroviral plasmids
Kit Components	NFATC2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 4773). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq	NM_001136021, NM_001258292, NM_001258294, NM_001258295, NM_001258296, NM_001258297, NM_012340, NM_173091, NM_173091.1, NM_173091.2, NM_173091.3, NM_012340.1, NM_012340.2, NM_012340.3, NM_001136021.1, NM_001136021.2, NM_001258294.1, NM_001258296.1, NM_001258295.1, NM_001258297.1, NM_001258292.1, BC136418, BC144074, BM463165, NM_001136021.3, NM_001258295.2, NM_001258296.2, NM_001258294.2, NM_012340.5, NM_001258297.2, NM_001258292.2, NM_173091.4
Summary	This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.