HCK Human shRNA Plasmid Kit (Locus ID 3055)

Specifications

Product Data
Product Name	HCK Human shRNA Plasmid Kit (Locus ID 3055)
Locus ID	3055
UniProt ID	P08631
Synonyms	JTK9; p59Hck; p61Hck
Vector	pRFP-C-RS
Format	Retroviral plasmids
Kit Components	HCK - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector(Gene ID = 3055). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRFP-C-RS Vector, TR30015, included for free.
RefSeq	NM_001172129, NM_001172130, NM_001172131, NM_001172132, NM_001172133, NM_002110, NM_002110.1, NM_002110.2, NM_002110.3, NM_001172131.1, NM_001172129.1, NM_001172133.1, NM_001172132.1, NM_001172130.1, BC094847, BC014435, BC073748, BC108930, BC108931, BC113854, BC114463, NM_001172130.2, NM_001172129.2, NM_001172131.2, NM_001172133.2, NM_001172132.2, NM_002110.4
Summary	The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.