Mesothelin (MSLN) Human shRNA Plasmid Kit (Locus ID 10232)

Specifications

Product Data
Product Name	Mesothelin (MSLN) Human shRNA Plasmid Kit (Locus ID 10232)
Locus ID	10232
UniProt ID	Q13421
Synonyms	MPF; SMRP
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	MSLN - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 10232). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001177355, NM_005823, NM_013404, NM_005823.1, NM_005823.2, NM_005823.3, NM_005823.4, NM_005823.5, NM_013404.1, NM_013404.2, NM_013404.3, NM_013404.4, NM_001177355.1, NM_001177355.2, BC003512, BC003512.1, BC009272, NM_001177355.3, NM_005823.6
Summary	This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.