Enpp2 Mouse shRNA Plasmid (Locus ID 18606)

Specifications

Product Data
Product Name	Enpp2 Mouse shRNA Plasmid (Locus ID 18606)
Locus ID	18606
UniProt ID	Q9R1E6
Synonyms	AT; ATX; Auto; E-NPP 2; lysoPLD; N; Npps2; Pd; PD-; PD-Ialpha; Pdnp2
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	Enpp2 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 18606). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	BC003264, BC058759, NM_001136077, NM_001285994, NM_001285995, NM_015744, NM_015744.1, NM_015744.2, NM_015744.3, NM_015744.4, NM_001136077.1, NM_001136077.2, NM_001136077.3, NM_001285994.1, NM_001285994.2, NM_001285995.1, NM_001285995.2
Summary	This gene encodes a member of the phosphodiesterase and nucleotide pyrophosphatase family of bifunctional enzymes that hydrolize phosphodiester bonds of various nucleotides. The encoded protein undergoes proteolytic processing to generate a mature protein with lysophospholipase D activity, catalyzing the cleavage of the choline group from lysophosphatidylcholine to produce lysophosphatidic acid. This gene is expressed in numerous tissues and participates in neural development, obesity, inflammation and oncogenesis. A complete lack of the encoded protein in mice results in aberrant vascular and neuronal development leading to embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature protein. [provided by RefSeq, Sep 2015]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.