Crem Rat shRNA Plasmid (Locus ID 25620)

Specifications

Product Data
Product Name	Crem Rat shRNA Plasmid (Locus ID 25620)
Locus ID	25620
UniProt ID	Q03061
Synonyms	Icer
Vector	pGFP-C-shLenti
Format	Lentiviral plasmids
Kit Components	Crem - Rat, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 25620). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq	NM_001110860, NM_001271101, NM_001271102, NM_001271245, NM_001271246, NM_001271247, NM_001271248, NM_017334, NM_001110860.1, NM_001110860.2, NM_017334.1, NM_017334.2, NM_001271248.1, NM_001271246.1, NM_001271102.1, NM_001271245.1, NM_001271247.1, NM_001271101.1, BC078899, NM_013086
Summary	Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Isoform Delta is an activator. Plays a role in spermatogenesis and is involved in spermatid maturation. Binding of isoform Tau (activator) to CRE is increased by CREB3L4. The CREM isoform Tau-CREB3L4 heterodimer functions through CRE and may recruit HIRA to CRE to regulate histone exchange (By similarity).[UniProtKB/Swiss-Prot Function]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.