UBP43 (USP18) Human shRNA Plasmid Kit (Locus ID 11274)
CAT#: TR300628
USP18 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
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CNY 6,790.00
货期*
2周
规格
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Specifications
Product Data | |
Product Name | UBP43 (USP18) Human shRNA Plasmid Kit (Locus ID 11274) |
Locus ID | 11274 |
UniProt ID | Q9UMW8 |
Synonyms | ISG43; PTORCH2; UBP43 |
Vector | pRS |
Format | Retroviral plasmids |
Kit Components | USP18 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 11274). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free. |
RefSeq | NM_017414, NM_017414.1, NM_017414.2, NM_017414.3, BC014896, BC014896.1, NM_017414.4 |
Summary | The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15. [provided by RefSeq, Sep 2011] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
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