RIC3 Human shRNA Plasmid Kit (Locus ID 79608)

Specifications

Product Data
Product Name	RIC3 Human shRNA Plasmid Kit (Locus ID 79608)
Locus ID	79608
UniProt ID	Q7Z5B4
Synonyms	AYST720; PRO1385
Vector	pRS
Format	Retroviral plasmids
Kit Components	RIC3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 79608). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001135109, NM_001206671, NM_001206672, NM_024557, NR_045405, NM_001346690, NM_001346691, NM_001346692, NM_001346693, NM_001346694, NR_144484, NR_144485, NR_144497, NM_024557.1, NM_024557.2, NM_024557.3, NM_024557.4, NM_024557.5, NM_001135109.1, NM_001135109.2, NM_001135109.3, NM_001206672.1, NM_001206672.2, NM_001206672.3, NM_001206671.1, NM_001206671.2, NM_001206671.3, BC022455, BC022455.1, NM_001206671.4, NM_001135109.4, NM_001206672.4
Summary	This gene encodes a member of the resistance to inhibitors of cholinesterase 3-like family which functions as a chaperone of specific 5-hydroxytryptamine type 3 receptor and nicotinic acetylcholine receptor subtypes. The encoded protein influences the folding and assembly of these receptor subunits in the endoplasmic reticulum and expression on the cell surface. This protein contains an N-terminal transmembrane domain, a proline-rich spacer, and a cytosolic C-terminal coiled-coil domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.