Constitutive androstane receptor (NR1I3) Human shRNA Plasmid Kit (Locus ID 9970)

Specifications

Product Data
Product Name	Constitutive androstane receptor (NR1I3) Human shRNA Plasmid Kit (Locus ID 9970)
Locus ID	9970
UniProt ID	Q14994
Synonyms	CAR; CAR1; MB67
Vector	pRS
Format	Retroviral plasmids
Kit Components	NR1I3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 9970). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	NM_001077469, NM_001077470, NM_001077471, NM_001077472, NM_001077473, NM_001077474, NM_001077475, NM_001077476, NM_001077477, NM_001077478, NM_001077479, NM_001077480, NM_001077481, NM_001077482, NM_005122, NM_001077480.1, NM_001077480.2, NM_001077470.1, NM_001077470.2, NM_001077473.1, NM_001077473.2, NM_001077476.1, NM_001077476.2, NM_001077479.1, NM_001077479.2, NM_001077481.1, NM_001077481.2, NM_001077482.1, NM_005122.1, NM_005122.2, NM_005122.3, NM_005122.4, NM_001077469.1, NM_001077469.2, NM_001077471.1, NM_001077471.2, NM_001077472.1, NM_001077472.2, NM_001077474.1, NM_001077474.2, NM_001077475.1, NM_001077475.2, NM_001077477.1, NM_001077477.2, NM_001077478.1, NM_001077478.2, BC069626, BC069626.1, BC121120, BC121121, BC030972, BC069651, NM_001077477.3, NM_005122.5, NM_001077475.3, NM_001077472.3, NM_001077476.3, NM_001077470.3, NM_001077479.3, NM_001077478.3, NM_001077474.3, NM_001077469.3, NM_001077480.3, NM_001077473.3, NM_001077481.3, NM_001077471.3
Summary	This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. In addition to drug metabolism, the CAR protein is also reported to regulate genes involved in glucose metabolism, lipid metabolism, cell proliferation, and circadian clock regulation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2020]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.