Gdf1 Mouse shRNA Plasmid (Locus ID 14559)

Specifications

Product Data
Product Name	Gdf1 Mouse shRNA Plasmid (Locus ID 14559)
Locus ID	14559
UniProt ID	P20863
Synonyms	AI385651; Gdf-1
Vector	pRS
Format	Retroviral plasmids
Kit Components	Gdf1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 14559). 5µg purified plasmid DNA per construct 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq	BC079555, NM_001163282, NM_008107, NM_001163282.1, NM_001163282.2, NM_008107.1, NM_008107.2, NM_008107.3, NM_008107.4, BC094351
Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. This protein is translated from a monocistronic mRNA early in development, and from a bicistronic mRNA in later stages that also encodes ceramide synthase 1. [provided by RefSeq, Jul 2016]
shRNA Design	These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed	OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.