PPP1A (PPP1CA) Human siRNA Oligo Duplex (Locus ID 5499)
CAT#: SR303671
PPP1CA (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
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CNY 4,090.00
货期*
4周
规格
Cited in 2 publications. |
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经常一起买 (3)
PPP1CA mouse monoclonal antibody, clone OTI6E5 (formerly 6E5)
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Purity | HPLC purified |
Quality Control | Tested by ESI-MS |
Sequences | Available with shipment |
Stability | One year from date of shipment when stored at -20°C. |
# of transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Note | Single siRNA duplex (10nmol) can be ordered. |
Reference Data | |
RefSeq | NM_001008709, NM_002708, NM_206873 |
Synonyms | PP-1A; PP1A; PP1alpha; PPP1A |
Components | PPP1CA (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 5499)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml |
Summary | The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). This broadly expressed gene encodes the alpha subunit of the PP1 complex that associates with over 200 regulatory proteins to form holoenzymes which dephosphorylate their biological targets with high specificity. PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies suggest that PP1 is an important regulator of cardiac function and that PP1 deregulation is implicated in diabetes and multiple types of cancer. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2020] |
Performance Guranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). |
Citations (2)
The use of this RNAi has been cited in the following citations: |
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CDK1-mediated phosphorylation at H2B serine 6 is required for mitotic chromosome segregation
,Seibert, M;Krüger, M;Watson, NA;Sen, O;Daum, JR;Slotman, JA;Braun, T;Houtsmuller, AB;Gorbsky, GJ;Jacob, R;Kracht, M;Higgins, JMG;Schmitz, ML;,
J. Cell Biol.
,PubMed ID 30765437
[PPP1CA]
|
MYC dephosphorylation by the PP1/PNUTS phosphatase complex regulates chromatin binding and protein stability
,Dingar, D;Tu, WB;Resetca, D;Lourenco, C;Tamachi, A;De Melo, J;Houlahan, KE;Kalkat, M;Chan, PK;Boutros, PC;Raught, B;Penn, LZ;,
Nat Commun
,PubMed ID 30158517
[PPP1CA]
|
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