PKC alpha (PRKCA) Human siRNA Oligo Duplex (Locus ID 5578)
CAT#: SR303735
PRKCA (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
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CNY 4,090.00
货期*
4周
规格
Cited in 2 publications. |
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经常一起买 (3)
PRKCA mouse monoclonal antibody, clone OTI5F4
CNY 1,999.00
CNY 3,600.00
Specifications
Product Data | |
Purity | HPLC purified |
Quality Control | Tested by ESI-MS |
Sequences | Available with shipment |
Stability | One year from date of shipment when stored at -20°C. |
# of transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Note | Single siRNA duplex (10nmol) can be ordered. |
Reference Data | |
RefSeq | NM_002737 |
Synonyms | AAG6; PKC-alpha; PKCA; PKCalpha; PKCI+/-; PRKACA |
Components | PRKCA (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 5578)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml |
Summary | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008] |
Performance Guranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). |
Citations (2)
The use of this RNAi has been cited in the following citations: |
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The Syndecan-4/Protein Kinase Ca Pathway Mediates Prostaglandin E2-induced Extracellular Regulated Kinase (ERK) Activation in Endothelial Cells and Angiogenesis in Vivo
,Federico Corti, Federica Finetti, Marina Ziche, and Michael Simons,
J. Biol. Chem., May 2013; 288: 12712 - 12721.
,PubMed ID 23525101
[PRKCA]
|
Protein Kinases D2 and D3 Are Novel Growth Regulators in HCC1806 Triple-negative Breast Cancer Cells
,QIN HAO, RAYMOND MCKENZIE, HUACHEN GAN, and HUA TANG,
Anticancer Res, Feb 2013; 33: 393 - 399.
[PRKCA]
|
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