ST18 Human siRNA Oligo Duplex (Locus ID 9705)

Specifications

Product Data
Purity	HPLC purified
Quality Control	Tested by ESI-MS
Sequences	Available with shipment
Stability	One year from date of shipment when stored at -20°C.
# of transfections	Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
Note	Single siRNA duplex (10nmol) can be ordered.
Reference Data
RefSeq	NM_014682, NM_001352826, NM_001352827, NM_001352828, NM_001352829, NM_001352830, NM_001352831, NM_001352832, NM_001352833, NM_001352834, NM_001352835, NM_001352836, NM_001352837, NM_001352838, NM_001352839, NM_001352840, NM_001352841, NM_001352842, NM_001352843, NM_001352844, NM_001352845, NM_001352846, NM_001352847, NM_001352848, NM_001352849, NM_001352850, NM_001352851, NM_001352852, NM_001352853, NM_001352854, NM_001352855, NM_001352856, NM_001352857, NM_001352858, NM_001352859, NM_001352860, NM_001352861, NM_001352862, NM_001352863, NM_001352864, NM_001352865, NM_001352866, NM_001352867, NM_001352868, NM_001352869, NM_001352870, NM_001352871, NM_001352872, NM_001352873, NM_001352874, NM_001352875, NM_001352876, NM_001352877, NM_001352878, NM_001352879, NM_001352880, NM_001352881, NM_001352882, NM_001352883, NM_001352884, NR_148195, NR_148196
Synonyms	NZF-3; NZF3; ZC2H2C3; ZC2HC10; ZNF387
Components	ST18 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 9705) Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol Included - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Summary	Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells.[UniProtKB/Swiss-Prot Function]
Performance Guranteed	OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.